CV - PEIRS Cedric

Name:

Dr. Cedric Peirs

Nationality:

France

Position:

Researcher Inserm CRCN

Education:

Ph.D. in Neuroscience, University of Auvergne (France).

M.Sc. in Cell Biology and Pathophysiology, University of Bordeaux (France) – University of Melbourne (Australia).

B.Sc. in Cell Biology and Physiology, University of Bordeaux (France).

Email:

cedric.peirs@inserm.fr

Address:

INSERM U1107 - Neuro-Dol: Trigeminal Pain and Migraine

Faculté de Chirurgie Dentaire

2, rue de Braga

63100, Clermont-Ferrand - FRANCE

Phone:

+33 4 73 17 73 11

Research Interest:

My research focusses on the neurobiology of pain, especially the neuronal circuitry of the spinal cord and medullary dorsal horns underlying chronic pain. I received a M.Sc. at the University of Bordeaux (France) and a Ph.D. in Neuroscience at the University of Auvergne (France). I did a postdoctoral training first with Dr. Rebecca Seal at the University of Pittsburgh (USA) and then with Pr. Radhouane Dallel at the University Clermont-Auvergne (France). I became a full researcher at the National Institute of Health and Medical Research (Inserm) in 2018.

Major contributions include 1) the investigation of the segmental circuit underlying static mechanical allodynia using the capsaicin pain model, 2) the identification of a cellular mechanism responsible for the disinhibition of PKCγ neurons by recative oxygen species (ROS) that leads to the transformation of touch into pain, 3) the identification of several neuronal populations that participate in the persistent pain network and 4) the concept that multiple spinal microcircuits encode mechanical hypersensitivity with their own signature role in facilitating the pain depending on the type of insult.

Currently, we combine opto/pharmacogenetics, behavior analysis, patch-clamp electrophysiology, cell biology, viral tracings and 2-photon calcium imaging to investigate the anatomical and functional organization of the neuronal circuits engaged in chronic pain, including those in the dorsal horn, but also their dynamics and the conditions of their recruitment.

Technical expertise:

Electrophysiology: Ex vivo whole cell patch-clamp recording (spinal cord slices with dorsal roots, DRGs and skin attached), optogenetic and pharmacogenetic, single cell morphology by biocytin tracing.

Surgery: French degree in animal experimentation Level 1, intra-spinal microinjection, cannulation and cerebral microinjection, intrathecal/intracisternal injections.

Imaging: Single to quadruple immunohistochemistry/immunofluorescence by confocal, multiphoton and electron microscopy, calcium imaging.

Cell Biology: Viral manipulation of spinal cord neurons, cell-specific transynaptic anterograde and retrograde tracings.

Molecular Biology: Analysis of genomic expression by polymerase chain reaction (PCR), semi-quantitative analysis of protein expression in cellular culture and tissue by Western blot using chemiluminescence/photofluorescence.

Behavior: Analysis of pain hypersensibility, food intake and locomotor activity in rats and mice, optogenetic and pharmacogenetics. 

Recent Publications:

1. Peirs C and Seal RP: Neural circuits for pain: Recent advances and current views. Science, 2016 Nov 4;354(6312):578-584. (IF = 34.7)

2. Peirs C, Bourgeois N, Artola A, Dallel R: PKCγ Interneurons Mediate C-Fiber-Induced Orofacial Secondary Static Mechanical Allodynia, but not C-Fiber-Induced Nociceptive Behavior. Anesthesiology, 2016 May 19;124(5):1136-52. (IF = 5.2)

3. Peirs C and Seal RP: Targeting Toll-like receptors to treat chronic pain. Nature Medicine, 2015 Nov 5;21(11):1251-2. (IF = 30.4)

4. Peirs C, Williams SP, Zhao X, Walsh CE, Gedeon JY, Cagle NE, Goldring AC, Hioki H, Liu Z, Marell PS, Seal RP: Dorsal Horn Circuits for Persistent Mechanical Pain. Neuron, 2015 Aug 19;87(4):797-812. (IF = 14.0)

5. Alba-Delgado C, El Khoueiry C, Peirs C, Dallel R, Artola A, Antri M: Subpopulations of PKCγ interneurons within the medullary dorsal horn revealed by electrophysiologic and morphologic approach. Pain, 2015 Sep;156(9):1714-28. (IF = 5.2)

6. Peirs C, Patil S, BoualiBenazouz R, Artola A, Landry M, Dallel R: Protein kinase C gamma interneurons in the rat medullary dorsal horn: distribution and synaptic inputs of these neurons, and subcellular localization of the enzyme. Journal of Comparative Neurology, 2014 Feb 1;522(2):393-413. (IF = 3.3)

7. Miraucourt LS, Peirs C, Dallel R, Voisin DL: Glycine inhibitory dysfunction turns touch into pain through astrocyte-derived D-serine. Pain, 2011 Jun;152(6):1340-8. (IF = 5.2)

Complete List of Published Work in My Bibliography:

https://www.ncbi.nlm.nih.gov/sites/myncbi/10Glow0pGDyQi/bibliography/40319463/public/?sort=date&direction=descending